Reply: Are IgG anti-DNA Secreting Hybridomas Representative?
نویسندگان
چکیده
منابع مشابه
Preferential production of IgM-secreting hybridomas by immunization with DNA vaccines coding for Ebola virus glycoprotein: use of protein boosting for IgG-secreting hybridoma production
PURPOSE The goal of this study was to investigate the utility of DNA vaccines encoding Ebola virus glycoprotein (GP) as a vaccine type for the production of GP-specific hybridomas and antibodies. MATERIALS AND METHODS DNA vaccines were constructed to express Ebola virus GP. Mice were injected with GP DNA vaccines and their splenocytes were used for hybridoma production. Enzyme-linked immunoso...
متن کاملConstruction of T cell hybridomas secreting allogeneic effect factor
T cell hybridoma lines were constructed by fusion of DBA/2 alloantigen-activated T cell blasts with the AKR thymoma line BW5147. Certain of the hybridomas prepared in this manner secreted spontaneously into their culture supernates biologically active molecules that displayed B cell- and T cell-activating properties characteristic of allogeneic effect factor (AEF). Cell surface phenotype analys...
متن کاملCharacterization of human hybridomas secreting antibody to tetanus toxoid.
We have selected a thioguanine-resistant lymphoblastoid cell line (LTR228) that forms human-human hybrids with high efficiency. Fusions with peripheral B cells consistently yield one colony per 10(5) cells plated. To produce antitetanus monoclonal antibodies, we withdrew blood from persons who had recently received booster injections of tetanus toxoid. T cells were separated from peripheral mon...
متن کاملARE IgG-ANTI-FAB2ɤ AND IgG-ANTI-HINGE ANTIBODIES ACTIVE CONSTITUENTS OF INTRAVENOUS IMMUNOGLOBULIN?
Our aim was to find out if immunoglobulins for intravenous use (IVIG) contain IgG-anti-F(ab')2γ (AFab2) and IgG-antiHinge (AH) antibodies (Ab.) and to discuss how AFab2, in particular AH could contribute to immunomodulatory effect of IVIGs. We measured IgG, AFab2 and AH in five IVIG products approved for therapeutic use. IVIG showed higher AFab2 and AH concentration than sera of randomized heal...
متن کاملCLONAL ANALYSIS OF IgG - SECRETING CELLS
The switch from IgM to IgG (or IgA, IgE) synthesis has been studied in both normal and neoplastic Ig-secreting cells (1-6). Evidence suggests that DNA rearrangements precede isotype switching. The nature of the signals that induce isotype switching are not understood, although T cells (7-13), T cell-derived lymphokines (14-20), and mitogens (20-22) can influence switching in activated B cells. ...
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ژورنال
عنوان ژورنال: Arthritis & Rheumatism
سال: 1994
ISSN: 0004-3591,1529-0131
DOI: 10.1002/art.1780370128